mab41441  (Bio-Techne corporation)

Journal: F1000Research

Article Title: Identification of high-performing antibodies for Apolipoprotein E for use in Western Blot and immunoprecipitation

doi: 10.12688/f1000research.133899.3

Figure Lengend Snippet: Summary of the Apolipoprotein E antibodies tested.

Article Snippet: Bio-Techne , MAB41441 , ZRQ0318021 , AB_2289763 , monoclonal , recombinant fragment , 395004 , rat , 5.0 , Wb.

Techniques: Concentration Assay, Recombinant

Journal: F1000Research

Article Title: Identification of high-performing antibodies for Apolipoprotein E for use in Western Blot and immunoprecipitation

doi: 10.12688/f1000research.133899.3

Figure Lengend Snippet: HAP1 WT and APOE KO were cultured in serum free media. Media were collected, concentrated, and 30 μg of protein were processed for Western Blot with the indicated Apolipoprotein E antibodies. The Ponceau stained transfers of each blot are shown. Antibody dilutions were chosen according to the recommendations of the antibody supplier. Exceptions were given for antibodies ab1907*,13366**,18254-1-AP, MA5-15852* and 66830-1-Ig* which were titrated to the concentrations listed below, as the signals were too weak when following the supplier’s recommendations. Antibody dilutions used: GTX635889* at 1/200, GTX635891* at 1/200, ab52607** at 1/200, ab51015** at 1/1000, ab1907* at 1/200, 13366** at 1/500, ARP54283 at 1/1000, 701241** at 1/200, MA5-41148** at 1/200, MA5-15852* at 1/200, MAB41441* at 1/200, NB110-60531* at 1/200, 18254-1-AP at 1/200, 66830-1-Ig* at 1/200. Apolipoprotein E predicted band size: 36 kDa. *Monoclonal antibody, **Recombinant antibody.

Article Snippet: Bio-Techne , MAB41441 , ZRQ0318021 , AB_2289763 , monoclonal , recombinant fragment , 395004 , rat , 5.0 , Wb.

Techniques: Cell Culture, Western Blot, Staining, Recombinant

Journal: bioRxiv

Article Title: APOE Impacts Lipid Trafficking in Retinal Pigment Epithelium Cells

doi: 10.1101/2024.05.30.596647

Figure Lengend Snippet: A) Example of donor eye tissue from an aged individual without AMD pathology. APOE is present in the neural retina (NR), Bruch’s membrane (BM), and choriocapillaris (CH) but not in the Retinal Pigment Epithelium (RPE). B) Example of donor eye tissue from an aged individual with intermediate AMD pathology. Soft drusen underlying the RPE layer (black arrows) are strongly positive for APOE throughout their mass. C) Example of donor eye tissue from an aged individual with intermediate AMD pathology. APOE accumulates around, but not inside, hard drusen (black arrows), small drusen with clearly defined boarders. For all panels, area in square outline is the high magnification image in the right subpanel.

Article Snippet: Primary antibodies for APOE (1:500; R&D Systems, MAB41441), ZO-1 (1:200; Invitrogen 33-9106) and CRALBP (1:500, Abcam, ab243664) were added overnight in blocking buffer at 4 °C with gentle shaking.

Techniques: Membrane

Journal: bioRxiv

Article Title: APOE Impacts Lipid Trafficking in Retinal Pigment Epithelium Cells

doi: 10.1101/2024.05.30.596647

Figure Lengend Snippet: A) Diagram of CRISPR editing strategy for APOE knockout and APOE isoform human iPSCs and iPS-RPE cells. B) Representative brightfield images of APOE iPS-RPE cells at 8 weeks in culture. Note severe pigment and morphological deficits in APOE KO (lower right) C) Representative images of ZO-1 staining in 8-week-old APOE iPS-RPE cells. Scale bar is 50µm. D) Cell surface area assessed from maximum intensity projections of phalloidin stained 8-week-old APOE iPS-RPE cells. E) Transepithelial electrical resistance (TEER) measured in 8-week-old APOE iPS-RPE cells and commonly used human iPS-RPE lines (01434 and FujiFilm iCell). * used to indicate any p <0.05 for simplicity.

Article Snippet: Primary antibodies for APOE (1:500; R&D Systems, MAB41441), ZO-1 (1:200; Invitrogen 33-9106) and CRALBP (1:500, Abcam, ab243664) were added overnight in blocking buffer at 4 °C with gentle shaking.

Techniques: CRISPR, Knock-Out, Staining

Journal: bioRxiv

Article Title: APOE Impacts Lipid Trafficking in Retinal Pigment Epithelium Cells

doi: 10.1101/2024.05.30.596647

Figure Lengend Snippet: A) Example images of APOE3 (left) and APOE KO (right) iPS-RPE cells. Scale bar indicates 650µm. B) Example images of CRALBP staining in 8wk old APOE KO, APOE2, APOE3, and APOE4 iPS-RPE cells. Scale bar indicates 20µm. C) Violin plots of cell surface area (µm 2 ). Heavy dashed line indicates median. Light dashed lines indicate upper and lower quartiles. D) Average APOE transcripts per million (TPM) detected in 4wk old APOE iPS-RPE. Data selected from bulk RNA sequencing described in .

Article Snippet: Primary antibodies for APOE (1:500; R&D Systems, MAB41441), ZO-1 (1:200; Invitrogen 33-9106) and CRALBP (1:500, Abcam, ab243664) were added overnight in blocking buffer at 4 °C with gentle shaking.

Techniques: Staining, RNA Sequencing